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SYSMEX ANALYZER INTERFACING GUIDE

Sysmex CA-600 / CS-2500 / CN-3000 Bi-Directional LIS Interfacing Guide

Complete engineering specification, HL7/ASTM protocols, pinouts, and parameter mapping for Sysmex CA-600 / CS-2500 / CN-3000 on AILS Cloud LIMS.

Sysmex CA-600 / CS-2500 / CN-3000 Analyzer - AILS LIS Interfacing Guide
Manufacturer
Sysmex
Protocol
ASTM E1394 / HL7
Interface Mode
Bi-Directional
Throughput
60 - 180 tests/hour

1. Instrument Overview & Interfacing Architecture

The Sysmex CA-600 / CS-2500 / CN-3000 is a high-performance coagulation instrument designed for diagnostic laboratories and hospital health networks. Integrating this analyzer directly with AILS Cloud LIMS eliminates manual transcription delays, prevents typographical patient identification errors, and automatically enforces Westgard delta check algorithms.

Bi-Directional Order Push

AILS automatically generates worklist queries (QRY^Q02 / ASTM orders) mapped to patient accession barcodes and pushes test instructions directly to the instrument sample tray.

Real-Time Result Ingestion

Raw OBX / Result records are parsed instantly within 200 milliseconds, flagged for abnormal reference ranges, and linked to pathologist digital signature queues.

2. Hardware Connection & Communication Settings

// Physical & Socket Configuration
TCP/IP Ethernet (Port 5001) / RS-232 DB9 Serial Cable (9600 Baud, 8-N-1).
// Protocol: ASTM E1394 / HL7 | Connection Type: TCP/IP (Port 5001) / RS-232 Serial

3. Sample Message Wire Frame

Below is a real-world communication frame transmitted between the Sysmex CA-600 / CS-2500 / CN-3000 and AILS LIS middleware:

<STX>1H|\^&|||Sysmex CA-600|||||||P|1394-97|20260815164000<CR><ETX>4C<CR><LF>
<STX>2P|1||||Walker^James|||M<CR><ETX>2A<CR><LF>
<STX>3O|1|ACC2026081558||^^^COAG_PANEL|||||||A<CR><ETX>4D<CR><LF>
<STX>4R|1|^^^PT|12.4|sec|11.0-13.5|N||F<CR><ETX>1B<CR><LF>
<STX>5R|2|^^^INR|1.02|Ratio|0.8-1.2|N||F<CR><ETX>2C<CR><LF>
<STX>6R|3|^^^APTT|29.5|sec|25.0-35.0|N||F<CR><ETX>28<CR><LF>
<STX>7R|4|^^^FIB|310.0|mg/dL|200.0-400.0|N||F<CR><ETX>3E<CR><LF>
<STX>8R|5|^^^D_DIMER|0.32|ug/mL|0.0-0.5|N||F<CR><ETX>25<CR><LF>
<STX>9L|1|N<CR><ETX>0A<CR><LF>

4. Parameter & LOINC Standard Code Mapping

Instrument Code LOINC Code Clinical Parameter Name Standard Units Default Reference Range
PT 5902-2 Prothrombin Time sec 11.0 - 13.5
INR 6301-6 International Normalized Ratio (INR) Ratio 0.8 - 1.2
APTT 3173-2 Activated Partial Thromboplastin Time sec 25.0 - 35.0
FIB 3255-7 Fibrinogen (Clauss Method) mg/dL 200.0 - 400.0
D_DIMER 48065-7 D-Dimer Quantitative (FEU) ug/mL 0.0 - 0.5

5. Biomedical Engineering Tips & Common Gotchas

1

In Sysmex CA/CS setup, configure Host Protocol to ASTM E1394 with Baud Rate 9600 or TCP Port 5001.

2

Verify that ISI (International Sensitivity Index) calibration value is synchronized for accurate INR calculation.

3

Optical reaction curve analysis data (scattered light intensity) can be stored for clot reaction verification.

TECHNICAL FAQ

Frequently Asked Questions: Sysmex CA-600 / CS-2500 / CN-3000 Interfacing

Common biomedical engineering questions regarding connection, worklist setup, and data capture with AILS Cloud LIMS.

Q: What interface protocols are supported by Sysmex CA-600 and CS-2500?

Sysmex CA-600 and CS series coagulation analyzers use ASTM E1381/E1394 standard protocol over RS-232 serial or TCP/IP socket.

Q: How does AILS handle INR calculations from Prothrombin Time results?

AILS verifies the measured PT in seconds against the analyzer's mean normal PT and reagent ISI factor, confirming the transmitted INR value.

Q: How are critical panic values (e.g. INR > 4.5 or Fibrinogen < 100 mg/dL) handled?

Critical coagulation values trigger visual panic alerts on laboratory dashboards and send automated emergency SMS alerts to clinicians.

Q: Can CS-2500 multi-wavelength optical pre-analytical checks (HIL) be captured?

Yes. Pre-analytical sample integrity checks for Hemolysis, Icterus, and Lipemia are logged to assess potential coagulation interference.

Q: What serial cable is required for Sysmex CA-600?

A standard DB9 Female Null Modem serial cable running at 9600 baud, 8 data bits, Parity None, 1 stop bit.

PRE-CONFIGURED DRIVER READY

Connect Your Sysmex CA-600 / CS-2500 / CN-3000 to AILS in Under 15 Minutes

Our specialized biomedical engineering team provides complete plug-and-play drivers, cable diagrams, and bi-directional test verification for the Sysmex CA-600 / CS-2500 / CN-3000.