Sysmex UF-4000 / UF-5000 Bi-Directional LIS Interfacing Guide
Complete engineering specification, HL7/ASTM protocols, pinouts, and parameter mapping for Sysmex UF-4000 / UF-5000 on AILS Cloud LIMS.
1. Sysmex UF-4000 / UF-5000 Interfacing Overview & Diagnostic Workflow
The Sysmex UF-4000 / UF-5000 is a precision diagnostic analyzer designed for clinical laboratory testing. Connecting the Sysmex UF-4000 / UF-5000 directly to AILS Cloud LIMS eliminates manual data entry, prevents typographical identification errors, and delivers verified results directly to ordering physicians and hospital EMRs.
AILS Gateway manages bi-directional ASTM E1394 / HL7 v2.5 worklist handshakes with the Sysmex UF-4000 / UF-5000, streaming patient test requests as samples are loaded.
Results are checked against age- and gender-adjusted reference ranges, delta check baselines, and quality control (QC) Levey-Jennings rules before digital sign-off.
2. Sysmex UF-4000 / UF-5000 TCP/IP Socket & Network Configuration
Ethernet RJ45 TCP/IP MLLP Socket (Port 5001). ASTM E1394 or HL7 v2.5.
[Instrument IP Client] [AILS Gateway Server] Socket Address -------------> Static IP : Port 5001 Start of Block -------------> <VT> ASCII 0x0B End of Block -------------> <FS><CR> ASCII 0x1C 0x0D Payload Standard -------------> ASTM E1394 / HL7 v2.5 KeepAlive Probe -------------> Bi-Directional ACK (AA)
3. Sysmex UF-4000 / UF-5000 HL7 v2.x Message Stream
Below is a validated communication frame transmitted between the Sysmex UF-4000 / UF-5000 and AILS LIS middleware:
<STX>1H|\^&|||Sysmex UF-5000|||||||P|1394-97|20260815170000<CR><ETX>4B<CR><LF> <STX>2P|1||||Sanchez^Elena|||F<CR><ETX>2A<CR><LF> <STX>3O|1|ACC2026081562||^^^URINE_SEDIMENT|||||||A<CR><ETX>5F<CR><LF> <STX>4R|1|^^^RBC|12.4|/uL|0.0-15.0|N||F<CR><ETX>1D<CR><LF> <STX>5R|2|^^^WBC|45.2|/uL|0.0-25.0|H||F<CR><ETX>2C<CR><LF> <STX>6R|3|^^^EC|8.0|/uL|0.0-10.0|N||F<CR><ETX>17<CR><LF> <STX>7R|4|^^^CAST|0.45|/uL|0.0-1.0|N||F<CR><ETX>2E<CR><LF> <STX>8R|5|^^^BACT|850.0|/uL|0.0-100.0|H||F<CR><ETX>34<CR><LF> <STX>9L|1|N<CR><ETX>0A<CR><LF>
4. Sysmex UF-4000 / UF-5000 Clinical Parameter LOINC & Reference Range Dictionary
| Instrument Code | LOINC Code | Clinical Parameter Name | Standard Units | Default Reference Range |
|---|---|---|---|---|
| RBC | 20448-7 | Red Blood Cells Quantitative | /uL | 0.0 - 15.0 |
| WBC | 20449-5 | White Blood Cells Quantitative | /uL | 0.0 - 25.0 |
| EC | 20450-3 | Epithelial Cells Quantitative | /uL | 0.0 - 10.0 |
| CAST | 20451-1 | Hyaline & Pathological Casts | /uL | 0.0 - 1.0 |
| BACT | 20452-9 | Bacteria Count (Bacteriuria) | /uL | 0.0 - 100.0 |
5. Sysmex Biomedical Engineering Field Notes & Gotchas
In UF-5000 IPU communication settings, configure Host Interface to ASTM E1394 over TCP Port 5001.
High bacterial counts (BACT > 100/uL) with elevated WBCs automatically flag suspected Urinary Tract Infection (UTI).
Dysmorphic RBC information (Non-Lysed RBC, Glomerular vs Non-Glomerular) is captured in dedicated clinical research fields.
Frequently Asked Questions: Sysmex UF-4000 / UF-5000 LIS Interfacing
Common biomedical engineering questions regarding connection, worklist setup, and data capture with AILS Cloud LIMS.
Q: What technology is used by Sysmex UF-5000 for urinalysis?
Sysmex UF-5000 uses blue semiconductor laser fluorescence flow cytometry to count and classify urinary formed elements (RBC, WBC, casts, epithelial cells, bacteria, yeast, and crystals) in quantitative units per microliter (/uL).
Q: How does UF-5000 transmit results to AILS LIMS?
UF-5000 transmits complete particle enumeration data and scattergram flags over TCP/IP socket (Port 5001) using ASTM E1394 or HL7 v2.5.
Q: How does AILS handle automated bacteriuria and UTI screening?
When BACT and WBC counts exceed clinical thresholds, AILS automatically flags the specimen for reflex urine culture and antibiotic susceptibility testing (AST).
Q: Can Sysmex UF-5000 connect alongside a urine test strip analyzer (e.g. UC-3500)?
Yes. The consolidated UN-Series urine workcell combines test strip chemistry and flow cytometry sediment analysis into a single unified patient report.
Q: How are abnormal casts and dysmorphic RBCs reviewed in AILS?
Pathological casts and abnormal red cell morphology flags automatically route the case to the pathologist microscopy review queue.
Related Urinalysis Instrument Guides
Compare protocols, pinouts, and parameter mapping dictionaries for comparable diagnostic equipment.
Roche Cobas u411 / Cobas 6500
High-throughput urine test strip reader and digital microscopy workcell. Automated physical and chemical urinalysis result capture into LIMS.
Sysmex XN-1000
High-throughput 5-part differential hematology analyzer. AILS LIMS receives complete CBC + DIFF parameters with automated flag mapping and delta check validation.
Sysmex XN-550 / XN-2000
Compact automated 5-part differential hematology analyzer with integrated auto-sampler. Bi-directional host query pulls test profiles by tube barcode.
Connect Your Sysmex UF-4000 / UF-5000 to AILS in Under 15 Minutes
Our specialized biomedical engineering team provides complete plug-and-play drivers, cable diagrams, and bi-directional test verification for the Sysmex UF-4000 / UF-5000.