Roche Cobas c501 / c502 / Integra 400 Bi-Directional LIS Interfacing Guide
Complete engineering specification, HL7/ASTM protocols, pinouts, and parameter mapping for Roche Cobas c501 / c502 / Integra 400 on AILS Cloud LIMS.
1. Roche Cobas c501 / c502 / Integra 400 Interfacing Overview & Diagnostic Workflow
The Roche Cobas c501 / c502 / Integra 400 is a high-performance clinical chemistry analyzer dedicated to routine photometric assays, enzymatic kinetics, and ion-selective electrode (ISE) electrolytes. Connecting the Roche Cobas c501 / c502 / Integra 400 to AILS Cloud LIMS establishes a closed-loop analytical cycle—automating test profile assignment, monitoring serum index flags (Hemolysis, Icterus, Lipemia), and preventing reporting errors across high-volume chemistry workloads.
As sample tubes enter the Roche Cobas c501 / c502 / Integra 400 reaction carousels, the onboard barcode reader queries AILS Gateway via ASTM E1394 / Roche Host Protocol to pull ordered test profiles (e.g. Liver Function, Renal Panel, Lipid Profile) in under 300ms.
AILS automatically reads HIL (Hemolysis, Icterus, Lipemia) interferent flags emitted by the Roche Cobas c501 / c502 / Integra 400, warning technologists when sample integrity compromises photometric accuracy and logging auto-dilution rerun factors.
2. Roche Cobas c501 / c502 / Integra 400 TCP/IP Socket & Network Configuration
TCP/IP Ethernet (Port 6000) / RS-232 Serial (19200/38400 Baud).
[Instrument DB9] [AILS Gateway PC] Pin 2 (TxD / Data Out) ---------> Pin 2 (RxD / Data In) Pin 3 (RxD / Data In) <--------- Pin 3 (TxD / Data Out) Pin 5 (GND / Signal) <========> Pin 5 (GND / Signal) Pin 7 (RTS) & Pin 8 (CTS) [Loopback or None] Baud: 19200 / 38400 | Data Bits: 8 | Parity: None | Stop: 1
3. Roche Cobas c501 / c502 / Integra 400 ASTM E1394 Record Frame
Below is a validated communication frame transmitted between the Roche Cobas c501 / c502 / Integra 400 and AILS LIS middleware:
<STX>1H|\^&|||Roche Cobas c501|||||||P|1394-97|20260815134000<CR><ETX>5F<CR><LF> <STX>2P|1||||Evans^Barbara|||F<CR><ETX>3D<CR><LF> <STX>3O|1|ACC2026081512||^^^CHEM_CORE|||||||A<CR><ETX>4E<CR><LF> <STX>4R|1|^^^GLUC|102.0|mg/dL|70.0-100.0|H||F<CR><ETX>1C<CR><LF> <STX>5R|2|^^^CREAT|0.95|mg/dL|0.6-1.2|N||F<CR><ETX>2A<CR><LF> <STX>6R|3|^^^NA|141.0|mmol/L|136.0-145.0|N||F<CR><ETX>18<CR><LF> <STX>7L|1|N<CR><ETX>0A<CR><LF>
4. Roche Cobas c501 / c502 / Integra 400 Clinical Parameter LOINC & Reference Range Dictionary
| Instrument Code | LOINC Code | Clinical Parameter Name | Standard Units | Default Reference Range |
|---|---|---|---|---|
| GLUC | 2345-7 | Glucose Fasting | mg/dL | 70.0 - 100.0 |
| CREAT | 2160-0 | Creatinine | mg/dL | 0.6 - 1.2 |
| NA | 2951-2 | Sodium ISE | mmol/L | 136.0 - 145.0 |
5. Roche Biomedical Engineering Field Notes & Gotchas
In Cobas Core software, configure Host Protocol to ASTM E1394 Mode 2 for bi-directional query.
Ensure rack barcode format matches the identification string configured in AILS LIMS.
Check that reagent cassette lot numbers are synchronized with QC validation tables.
Frequently Asked Questions: Roche Cobas c501 / c502 / Integra 400 LIS Interfacing
Common biomedical engineering questions regarding connection, worklist setup, and data capture with AILS Cloud LIMS.
Q: What interface standard is used by Roche Cobas c501 and cobas 6000 systems?
Roche Cobas systems utilize the Roche Host Interface (ASTM E1381/E1394) or HL7 v2.5 over TCP/IP socket (Port 6000) or RS-232 serial.
Q: How does Cobas c501 handle bi-directional sample worklist queries?
When a sample rack enters the loading line, the barcode reader sends a Q query. AILS Gateway responds with an O (Order) record containing requested test profiles.
Q: Can Cobas c501 chemistry and e601 immunoassay modules share one LIS connection?
Yes. Consolidated Cobas 6000 and 8000 core lab tracks connect via a single consolidated gateway interface.
Q: How are serum indices (Lipemia, Hemolysis, Icterus) handled in AILS?
Roche Serum Indices transmitted in comment or result records are linked to sample integrity flags in AILS LIMS.
Q: How are rerun and reflex tests scheduled on Cobas c501?
If a test result is out of linearity, AILS reflex rules can automatically command the Cobas to schedule an auto-diluted rerun.
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Connect Your Roche Cobas c501 / c502 / Integra 400 to AILS in Under 15 Minutes
Our specialized biomedical engineering team provides complete plug-and-play drivers, cable diagrams, and bi-directional test verification for the Roche Cobas c501 / c502 / Integra 400.