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ROCHE ANALYZER INTERFACING GUIDE

Roche Cobas c 111 / c 311 Bi-Directional LIS Interfacing Guide

Complete engineering specification, HL7/ASTM protocols, pinouts, and parameter mapping for Roche Cobas c 111 / c 311 on AILS Cloud LIMS.

Roche Cobas c 111 / c 311 Analyzer - AILS LIS Interfacing Guide
Manufacturer
Roche
Protocol
HL7 v2.5 / ASTM E1394
Interface Mode
Bi-Directional
Throughput
Up to 300 tests/hour

1. Roche Cobas c 111 / c 311 Interfacing Overview & Diagnostic Workflow

The Roche Cobas c 111 / c 311 is a high-performance clinical chemistry analyzer dedicated to routine photometric assays, enzymatic kinetics, and ion-selective electrode (ISE) electrolytes. Connecting the Roche Cobas c 111 / c 311 to AILS Cloud LIMS establishes a closed-loop analytical cycle—automating test profile assignment, monitoring serum index flags (Hemolysis, Icterus, Lipemia), and preventing reporting errors across high-volume chemistry workloads.

Bi-Directional Host Query & Rack Loading

As sample tubes enter the Roche Cobas c 111 / c 311 reaction carousels, the onboard barcode reader queries AILS Gateway via HL7 v2.5 / ASTM E1394 to pull ordered test profiles (e.g. Liver Function, Renal Panel, Lipid Profile) in under 300ms.

Serum Index & Auto-Dilution Reflux

AILS automatically reads HIL (Hemolysis, Icterus, Lipemia) interferent flags emitted by the Roche Cobas c 111 / c 311, warning technologists when sample integrity compromises photometric accuracy and logging auto-dilution rerun factors.

2. Roche Cobas c 111 / c 311 RS-232 Serial Port & Pinout Configuration

Hardware & Socket Configuration

TCP/IP Ethernet or RS-232 DB9 Serial. Host Port: 6000. Baud: 19200 or 38400, Parity: None, Data Bits: 8, Stop: 1.

Protocol: HL7 v2.5 / ASTM E1394
Interface Mode: Bi-Directional
Default Speed: 19200 / 38400
// RS-232 DB9 NULL-MODEM PINOUT SERIAL COM
[Instrument DB9]                   [AILS Gateway PC]
Pin 2 (TxD / Data Out) ---------> Pin 2 (RxD / Data In)
Pin 3 (RxD / Data In)  <--------- Pin 3 (TxD / Data Out)
Pin 5 (GND / Signal)   <========> Pin 5 (GND / Signal)
Pin 7 (RTS) & Pin 8 (CTS)        [Loopback or None]
Baud: 19200 / 38400 | Data Bits: 8 | Parity: None | Stop: 1
Verified with AILS Gateway Daemon & Real-Time Socket Router.

3. Roche Cobas c 111 / c 311 HL7 v2.x Message Stream

Below is a validated communication frame transmitted between the Roche Cobas c 111 / c 311 and AILS LIS middleware:

MSH|^~\&|COBAS-C311|Roche|AILS_LIMS|LAB_MAIN|20260815130000||ORU^R01|MSG009890|P|2.5
PID|1||P982375||Johnson^Robert||19651130|M
OBR|1|ORD982375|ACC2026081505|LIPID^Lipid Panel|||20260815125500
OBX|1|NM|CHOL2^Cholesterol Gen.2||215.0|mg/dL|140.0-200.0|H|||F
OBX|2|NM|TRIGL^Triglycerides||165.0|mg/dL|50.0-150.0|H|||F
OBX|3|NM|NA^Sodium ISE||140.5|mmol/L|136.0-145.0|N|||F

4. Roche Cobas c 111 / c 311 Clinical Parameter LOINC & Reference Range Dictionary

Instrument Code LOINC Code Clinical Parameter Name Standard Units Default Reference Range
CHOL2 2093-3 Total Cholesterol mg/dL 140.0 - 200.0
TRIGL 2571-8 Triglycerides mg/dL 50.0 - 150.0
NA 2951-2 Sodium ISE mmol/L 136.0 - 145.0

5. Roche Biomedical Engineering Field Notes & Gotchas

1

Cobas requires strict QRY^Q02 worklist handshake when operating in host-query mode.

2

Verify calibration curve lot numbers are registered before test run dispatch.

3

ISE channel test codes must match the specific Cobas channel numbering schema.

TECHNICAL FAQ

Frequently Asked Questions: Roche Cobas c 111 / c 311 LIS Interfacing

Common biomedical engineering questions regarding connection, worklist setup, and data capture with AILS Cloud LIMS.

Q: What interface protocol does Roche Cobas c311 use?

Roche Cobas c311 uses HL7 v2.5 or ASTM E1394 (Roche Host Interface protocol) over TCP/IP socket (Port 6000) or RS-232 serial.

Q: How does Cobas c311 communicate in Host-Query mode?

When a sample tube is placed on the carousel, the barcode reader queries AILS LIMS and receives test order profiles.

Q: How are STAT urgent samples prioritized on Cobas c311?

When a STAT order is sent from AILS LIMS with priority code S in the order segment, Cobas processes it immediately.

Q: How are serum indices (Lipemia, Hemolysis, Icterus) captured?

Serum indices transmitted in comment or result records are mapped to specimen integrity warnings in AILS LIMS.

Q: What baud rate is used for Cobas c111 serial interfacing?

Standard baud rate is 19200 or 38400 bps, 8 data bits, Parity None, 1 stop bit.

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