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HORIBA / ABX ANALYZER INTERFACING GUIDE

ABX Pentra 60C+ Bi-Directional LIS Interfacing Guide

Complete engineering specification, HL7/ASTM protocols, pinouts, and parameter mapping for ABX Pentra 60C+ on AILS Cloud LIMS.

ABX Pentra 60C+ Analyzer - AILS LIS Interfacing Guide
Manufacturer
Horiba / ABX
Protocol
MS Access ODBC / RS232
Interface Mode
Uni-Directional
Throughput
60 samples/hour

1. ABX Pentra 60C+ Interfacing Overview & Diagnostic Workflow

The ABX Pentra 60C+ is an automated hematology platform engineered for high-precision complete blood counts (CBC) and white blood cell differential analysis. In modern clinical laboratory workflows, interfacing the ABX Pentra 60C+ directly to AILS Cloud LIMS eliminates manual transcription delays, captures full WBC scattergram channel flags (such as immature granulocytes, atypical lymphocytes, and platelet clumps), and validates patient results against historical delta check baselines in real time.

Real-Time EDTA Tube Accessioning

AILS Gateway parses sample accession barcodes from the ABX Pentra 60C+ data stream, linking multi-parameter CBC results directly to open patient encounter records without manual intervention.

Cytometry Flagging & Delta Check Interlock

Raw result segments are evaluated instantly against laboratory reference intervals and Westgard multirule delta check algorithms. Critical alerts (e.g. severe thrombocytopenia or extreme leukocytosis) trigger automatic hold flags for pathologist microscopic smear review.

2. ABX Pentra 60C+ RS-232 Serial Port & Pinout Configuration

Hardware & Socket Configuration

MS Access ODBC Database Sync Agent / RS232 Serial Port (9600 Baud, 8-N-1).

Protocol: MS Access ODBC / RS232
Interface Mode: Uni-Directional
Default Speed: 9600 / ODBC
// RS-232 DB9 NULL-MODEM PINOUT SERIAL COM
[Instrument DB9]                   [AILS Gateway PC]
Pin 2 (TxD / Data Out) ---------> Pin 2 (RxD / Data In)
Pin 3 (RxD / Data In)  <--------- Pin 3 (TxD / Data Out)
Pin 5 (GND / Signal)   <========> Pin 5 (GND / Signal)
Pin 7 (RTS) & Pin 8 (CTS)        [Loopback or None]
Baud: 9600 / ODBC | Data Bits: 8 | Parity: None | Stop: 1
Verified with AILS Gateway Daemon & Real-Time Socket Router.

3. ABX Pentra 60C+ ASTM E1394 Record Frame

Below is a validated communication frame transmitted between the ABX Pentra 60C+ and AILS LIS middleware:

[ODBC_RESULT_POLL_RECORD]
SampleID: ACC2026081524
PatientName: Davis, Michael
RunTimestamp: 2026-08-15 14:20:12
WBC: 7.15 (10^3/uL)
RBC: 4.85 (10^6/uL)
HGB: 15.0 (g/dL)
PLT: 220 (10^3/uL)
Status: Validated

4. ABX Pentra 60C+ Clinical Parameter LOINC & Reference Range Dictionary

Instrument Code LOINC Code Clinical Parameter Name Standard Units Default Reference Range
WBC 6690-2 White Blood Cells 10*3/uL 4.0 - 11.0
RBC 789-8 Red Blood Cells 10*6/uL 4.5 - 5.9
HGB 718-7 Hemoglobin g/dL 13.5 - 17.5

5. Horiba / ABX Biomedical Engineering Field Notes & Gotchas

1

Ensure Horiba local data export utility is actively flushing completed runs to intermediate DB.

2

AILS background gateway polls table 'RESULTS' every 2000ms with transaction locking.

3

Verify that 5-part differential DHSS matrix data is exported.

TECHNICAL FAQ

Frequently Asked Questions: ABX Pentra 60C+ LIS Interfacing

Common biomedical engineering questions regarding connection, worklist setup, and data capture with AILS Cloud LIMS.

Q: How does ODBC integration work for Horiba Pentra 60C+?

AILS Gateway connects directly to the local MS Access (.mdb) database updated by the Pentra workstation, polling new completed runs automatically.

Q: Can Pentra 60C+ connect via RS-232 serial cable instead of ODBC?

Yes. Pentra 60C+ also supports direct serial COM output at 9600 baud using the standard ABX protocol.

Q: How frequently does AILS Gateway poll the Pentra 60C+ database?

The default polling interval is 2000 milliseconds, ensuring real-time result ingestion with zero server lag.

Q: Are 5-part differential parameters captured via ODBC?

Yes. Neutrophils, Lymphocytes, Monocytes, Eosinophils, and Basophils (# and %) are extracted from table records.

Q: What happens if the local computer restarts?

AILS Gateway runs as a resilient Windows service with automatic startup and crash recovery.

INSTRUMENT DIRECTORY

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